Hematopoietic stem cells induce immune tolerance to kidney transplantation in rats

Objective: To induce immune tolerance to allogenic kidney transplantation with hematopoietic stem cells（HSC） in recipient rats and to study its mechanism. Methods: A model of kidney transplantation was established.HSC were collected by StemSepTM.Wistar rats were treated with TBI and fed with CsA.HBC were injected into recipient rats and kidney transplantation was performed on the same day.The survival time of recipient rats was observed.Serum creatinine was measured and color Doppler flow imaging was checked.The immunologic tolerance status in rat renal allografts was detected by MLR on day 30,the recipient rat spleen cells were cultured with donor spleen cells as a third part. Results: The difference was significant between the case and other three control groups.Hypo-response to the donor occurred while the normal reaction to the third parity was retained. Conclusion: Hematopoietic stem cells can induce donor-specific tolerance to allografts.