Effect of different glucose concentrations on proliferation and apoptosis of pancreatic islet β-cells in mice

Objective To study the effect of different glucose concentrations on proliferation and apoptosis of pancreatic islet β-cells in mice. Methods Proliferation and apoptosis of MIN-6 cells were detected by MTT assay and cytometry,respectively with Annexin V-FITC staining after they were cultured with glucose at different concentrations（5.6,11.1,16.6,25.0 and 33.3mmol/L）for 72h. Results The proliferation rate of MIN-6 cells was significantly lower while the apoptosis rate of MIN-6 cells in mice was significantly higher 72h after they were cultured with glucose at the concentrations of 5.6,11.1 and 33.3mmol/L than at the concentration of 25mmol/L（P＜0.05）. Conclusion The proliferation of MIN-6 cells can be inhibited while the apoptosis of MIN-6 cells can be increased in mice by glucose at the concentrations of＜25mmol/L and＞25mmol/L.