Study of secretory αFGF gene transfected to cardiomyocytes in vitroand in vivo

Objective: To investigate the expression of constructed secretory αFGF gene in cardiomyocytes in vitro and in vivoand its angiogenic effect on host myocardium Methods: Secretory eucaryotic expression vector pCDNA3.1+SP αFGF was constructed and transfected in vitroto the neonate rat cardiomyocytes.The expression of αFGF was assessed.After transplantation of cells transfected with αFGF into the hearts of adult syngenic mice,the expression of αFGF in vivo and its angiogenic effect in host myocardium were studied. Results: The expression of αFGF was demonstrated by Western blotting and ELISA in the cultured media 24- 96 hours later. Using immunohistochemistry analysis, we detected αFGF in the recipient myocardium 96 hours later 20 days after cell transplantation, histological study revealed more blood vessels around the grafted cardiomyocytes in the host myocardium which was not marked in the controlled myocardium Conclusion: The plasmid we constructed can succeed in expressing and secreting αFGF peptidein vitroand in vivo,which would lead to angiogenesis in the recipient myocardiu