Concentration change of catecholamine and leucine-enkephalin in sheep cerebrospinal fluid after APA microencapsulated bovine chromaffin cells transplantation to the spinal subarachnoid space

Objective: To observe the change of catecholamine （CA） and leucine-enkephalin（L-EK） concentration in sheep cerebrospinal fluid after APA Microencapsulated Bovine Chromaffin Cells transplantation to the spinal subarachnoid space, to analyze the analgesic mechanism of transplantation and the immuno-protecting effect of APA-microcapsulation. Methods: To check concentration of norepinephrine（NE）, epinephrine（E） and leucine-enkephalin（L-EK） in sheep cerebrospinal fluid after APA Microencapsulated Bovine Chromaffin Cells（APA-BCCs）, BCCs and hollow microcapsule transplantation to the spinal subarachnoid space. Results: the concentration of E, NE and L-EK didn’t increased after transplantation of hollow microcapsule,increased after BCCs transplantation within 7 days and APA-BCCs transplantation within 28 days. Conclusion: BCCs transplantation to the spinal subarachnoid space could improve the concentration of CA and L-EK in host cerebrospinal fluid, the improvement may be related to analgesic effect, and APA-microencapsulation is good for lasting secretion of BCCs.