Screening and identification of genes trans-regulated by β-interferon protein with microarray assay

Objective: To screen genes regulated by IFN-βusing cDNA microarray technique. Methods: The IFN-βcoding DNA fragment was amplified with polymerase chain reaction（PCR） technique.The expressive vector of pcDNA3.1-IFN-βwas constructed by routine molecular biological methods.The HepG2 cells were transfected by pcDNA3.1（-） and pcDNA3.1-IFN-β respectively,using FuGENE6 transfection Reagent.The total RNA was isolated and reverse transcribed.The cDNAs were subjected for microarray screening with 1,152 cDNA probes. Results: The expressive vector has been constructed and confirmed by restriction enzyme digestion and DNA sequencing analysis.High quality mRNA and cDNA had been prepared and successful microarray screening had been conducted.From the scanning results,it was found 40 genes were up-regulated and 30 genes were down-regulated by IFN-β gene. Conclusion: IFN-βgene is a trans-regulator.