PSP57: A non-specific product of PSP94 mRNA variable splicing in prostatic tissues from patients with prostate carcinoma and benign prostatic hyperplasia

Objective: Analysize and compare the expression of human PSP94 and PSP57 mRNA in prostatic tissues from normal adults, patients with prostate carcinoma and benign prostatic hyperplasia （BPH）. Methods: Extract total RNA in prostatic tissues taken by operation from normal adults died of accident, patients with prostate carcionma and BPH, then do reverse transcriptase polymerase chain reaction （RT PCR）. RT PCR products are analysized by sequence determination and Southern blotting using specific exon Ⅱ of PSP94 and PSP57 and exon Ⅲ of PSP94 as probes. Results: The PSP94 and PSP57 mRNA are both found in the above three kinds of prostatic tissues. The cDNA sequences of PSP94 and PSP57 in normal prostatic tissue are consistent with that in carcinoma and hyperplasia tissues. Conclusion: PSP57 is a non specific product of PSP94 mRNA in prostatic tissues from patients with prostate carcinoma and benign prostatic hyperplasia.