Polymorphism of insulin receptor substrate 1 gene exon 1 in three Chinese native miniature pig strains

Objective To explore the polymorphism of insulin receptor substrate 1（IRS-1）in three strains of Chinese native miniature pigs（Zhongguonongda minipigs,Wuzhishan minipigs and Bama minipigs）in order to provide basic data for its use in metabolic disease and type 2 diabetes mellitus. Methods Genomic DNA was extracted from three strains of Chinese native miniature pigs,certain sequences of IRS-1 gene exon 1 were amplified by PCR.The amplified product was sequenced and identified.The polymorphism of IRS-1 gene was analyzed and compared with that of NCBI. Results Two SNPs were detected from the amplified fragment of Zhongguonongda miniature pig strains.The SNP of C2870G changed the amino acid from Ala to Gly on the locus of 956. Conclusion The two SNPs found in Zhongguonongda miniature pig strains but not in Bama and Wuzhishan miniature pig strains may be due to their genetic diversity.