Construction of human androgen receptor gene vectors with different CAG repeats by recombinant PCR

Objective: To construct human androgen receptor（hAR） gene vectors with different CAG repeats for expression in eukaryocytes.Methods: Middle fragments with 8,21,34 CAG repeats were amplified with corresponding leukocyte gene templates.Upstream and downstream fragments amplified with the plasmid template and cloned with wild type hAR gene were then spliced with the middle fragments to obtain whole target genes.Digested with SalⅠand NruⅠ,Target genes were inserted into the digested plasmid.Results: Restriction enzyme digestion and sequence analysis showed that the size and sequence of reconstructed plasmids with 8,21,34 CAG repeats were correct.Conclusion: The results indicate that recombinant PCR is an effective and feasible way for gene recombination in vitro.