Study on the interaction of estrogen receptor α and its target gene LRP16

Objective:LRP16 is an estrogen receptor（ERα） target gene,and the ERα-mediated transactivation is enhanced by LRP16.The study is to investigate the molecular mechanism underling this feedback effect.Methods: The in vitro and in vivo interactions of ERα and LRP16 were detected by GST-pulldown and coimmunoprecipitation（CoIP） assays,respectively.Results: LRP16 could directly bind to ERα in an estrogen-independent manner,but could be enhanced by estrogen.Conclusion: LRP16 is not only an estrogen-induced gene by ERα,but also acts as a coactivator of ERα.