Preparation and identification of keratin monoclonal antibody of tongue squamous cell carcinoma

Objective:To use tongue squamous cell carcinoma keratins as antigen to prepare the monoclonal antibody,and to evaluate the immunohistochemical location with the monoclonal antibody.Methods: Immunize BALB/c mice by B leukomonocyte hybridoma technique,five cloned strain had been acquired.18 cases of oral squamous cell carcinoma specimens were pathologically chosen and stained by S-P microwave immunohistochemical method.Results: Those five hybridoma cell strains which could secrete keratin monoclonal antibody of anti-tongue squamous cell carcinoma stably were named from Lck1 to Lck5.Lck1,Lck3,Lck4 and Lck5 could express partly in carcinoma tissue and metastasis lymphonode.Positive cells were located in cancer cells cytoplasm.Specificity and sensitivity of Lck4 were the highest.Conclusions: 5 strains of monoclonal antibody are acquired.Specificity and sensitivity of Lck4 are the highest in 5 strains of monoclonal antibody.