Cytotoxic activity of lymphocytes induceded by dendritic cells pulsed with tumor lysates antigen against mouse gastric-intestinal cancer in vitro

Objective: To observe the activity of dendritic cells（DC） against mouse gastric-intestinal cancer in vitro used DC pulsed with lysates of gastric-intestinal tumor cell（TAg） to induced murine spleen cells.Methods: DC,derived from murine bone marrow was induced by combination of GM-CSF,IL-4,and pulsed with TAg lysates of KATO3 and CT26 and then DCs were cocultured with spleen cells for 3d.And then these cytotoxic T cells（CTL） were used to kill the KATO3,CT26 and A375 target cells and the killer activities were detected by flow cytometry（FCM） and MTT method.Results: The biological activities of DC and CTL were detected well by FCM,and the cytotoxic activities of CTL induced by DC pulsed with TAg of KATO3 and CT26 were 75.98% and 86.75% to A375 target cells they were 10.57% and 11.53%.Conclusion: The CTL induced by DC pulsed with relative TAg has a significant specific cytotoxicity role to gastric-intestinal cancer target cell line cells in vitro.dendritic cell;cytotoxic T cell;gastric intestinal cancer;lysates antigen