Dependence of Mycobacterium tuberculosis on combined anti-tuberculosis drugs

Objective To investigate the dependence of clinical Mycobacterium tuberculosis （M. tuberculosis） isolating on combined anti-tuberculosis drugs. Methods Dependence test was performed for combined HR, HRS, and HRE in 184 strains of M. tuberculosis with double-phase Kuang’s agar medium. Results The dependence rate of M. tuberculosis for HR, HRS, and HRE was 13.04%（24/184）, 9.78%（18/184）, and 7.07%（13/184）, respectively. Of the 24 HR dependent strains, 17（75%） were R-dependent, 12（50%） were H-dependent, 7（29.17%） were H-and R-dependent, 1（4.17%） was not H-and R-dependent. Of the 18 HRS dependent strains, 17（94.44%） were R-dependent, 8 were H-dependent, 7（38.89%） were S-dependent, 3 were H-, R-and S-dependent, 1（5.56%） was not dependent on other drugs. Of the 13 HRE-dependent strains, 12（92.31%） were R-dependent, 6 （46.15%） were H-dependent, 10 （76.92%） were E-dependent, 4 （30.77%） were H-, R-and E-dependent. Strains which were not H-, R-and E-dependent were observed. Conclusion M.tuberculosis has a different extent of dependence on combined HR, HRS, and HRE, with the highest dependence on R.