Study on 19 kinds of lymphocyte phenotype before and after in vitro amplified from human peripheral blood lymphocyte

Objective: To study the 19 kinds of lymphocyte phenotype before and after in vitro amplified from 35 malignant tumor patients’ peripheral blood lymphocyte.Methods: The lymphocyte cells and mononuclear cells from peripheral blood of malignant tumor patients were induced and amplified,and the flow cytometry was used to detect the percentage of CD3⁺,etc.19 type cells in effector cells,and then their percentages before and after the in vitro amplification were compared.Results: After amplification,the percentage of CD3⁺,CD3⁺CD8⁺,CD3⁺CD16^-CD56⁺,CD3⁺CD16⁺CD56^-,CD3⁺CD16⁺CD56⁺,CD45RO⁺ and it's subgroups CD8⁺CD45RO⁺,CD8⁺CD28^-cells significantly increased（P＜0.01）;the percentage of CD19⁺,CD3⁺CD4⁺,CD3^-CD16^-CD56⁺,CD3^-CD16⁺CD56^-,CD3^-CD16⁺CD56⁺,CD45RA⁺ and it's subgroups CD4⁺CD45RA⁺,CD8⁺CD45RA⁺,CD4⁺CD45RO⁺,CD28⁺ and it's subgroup CD8⁺CD28⁺ cells significantly decreased（P＜0.01）.Conclusion: After amplification,the effector cells have the cytotoxic activity that can directly kill the tumor cells.