Experimental studies on xenotransplantation of microencapsulated rat islets into diabetic mice

Objective: To test immunoisolated effectiveness of microencapsulated rat islets as xenografts in diabetic mice.Methods: intraperitoneal xenotransplantation of micro encapsulated rat islets into kumin mice with the STZ-induced diabetes was conducted by Sun's electrostatic microencapsulated instrument and improving APA encapsulated method. Results: The diabetic state was reversed and normoglycemia was maintained for up to 110 days. The diabetic mice receiving transplats of unecapsulated islets experienced normoglycemia with 2~3 days. In contrast, the diabetic states were maintained in control groups of receiving empty capsules or 0.9% NaCl solution and diabetes group. The retrieved microcapsules in the 30th day posttransplantation were perfectly intact, spherical, smooth and poorly fibroblats on the capsules surface. The islets in the capsules were stained orange yellow with DTZ. Conclusion: These results demonstrated that Sun's microencapsulated preparation technique could prolong survival of rat pancreatic islets as xenografts, therefore this microencapsulation provide good immunoisolated effectiveness and biological appropriation.