The protective effect on hepatic ischemia/reperfusion injury by lidocaine injection into hepatoduodenal ligament in rats

Objective:To investigate the potential protective effect of lidocaine injected into hepatoduodenal ligament on hepatic I/R injury and its mechanism on the level of proteomics. Methods: 46 SD rats were randomly divided into 3 groups. I-R group: Animals were subjected to hepatic 40-minute I-R. Lidocaine group: Previous to hepatic 40 min I-R, 0.4% lidocaine hydrochloride was injected into hepatoduodenal ligament for 10 minutes. Laparotomy group: Laparotomy was performed without any treatments for 90 minutes. Blood samples of each group were processed to determine plasma alanine transaminase （ALT） and aspartate transaminase （AST）. Hepatic tissues were examined pathologically by trypan blue uptake of hepatocytes. Proteomic analysis of liver tissues were performed by two-dimensional polyacrylamide gel electrophoresis（2-D PAGE）and matrix-assisted laser desorption/ionization time of flight mass spectrometry （MALDI-TOF-MS）. Results: A significant decrease of ALT and AST was noted for lidocaine group VS I-R group（ALT: t=2.599, P＜0.02; AST: t=2.992, P＜0.01）. The protective effect of hepatocytes in lidocaine group was confirmed histologically by a marked reduction of nonviable hepatocytes with trypan blue uptake. Eleven proteins altered their expressions after the lidocaine pretreatment, of which six were up-expressed, five down-expressed. Conclusions: Previous to hepatic I-R, the pretreatment with lidocaine injected into hepatoduodenal ligament as a novel approach attenuates hepatic warm I-R injury of rats effectively, and alters some protein expressions of rat liver of I-R injury.