Study on replication deficient adenoviral vectors mediated enhanced green fluorescent protein transduction to neural stem cells

Objective:To study the feasibility and infection efficiency of replication deficient adenoviral vectors（AdVec） mediated enhanced green fluorescent protein（EGFP） transduction to neural stem cells（NSCs）.Methods: Rat's NSCs were cultured in vitro,AdVec-EGFP virus were amplified and purified.NSCs were transduced with AdVec-EGFP virus and results were estimated with fluorescence microscope and immnocytochemistry.Results: Report gene EGFP could be high performed in infected NSCs;fluorescein started to be expressed 6 hours after trasfected,and reached peak time afer 48 hours.The infection rate was（76±2）%;the biological characters of NSCs had no changes.Conclusions:AdVec-EGFP has higher gene expression efficiency of mediating exogenous gene;it is a better labelled compound for NSCs investigation both in vivo and in vitro.