N-cad-Fc chimera protein inhibits apoptosis accompanied by P19 differentiation process

Objective: To study the effects of N-cad-Fc on apoptosis accompanied by P19 differentiation process.Methods: N-cad-Fc was made through biomolecular technology.P19 differentiation was induced by retinoic acid（RA）,and P19 cells cultured on gelatin were used as control.Differentiated cells were observed with microscope.TURNEL method and DNA fragmentation analysis were used for apoptosis confirmation.Survival cells were detected by AlamarBlueTM method.Results: P19 cells cultured on N-cad-Fc coated dishes showed the same differentiation morphology to those cultured on gelatin coated dishes,but apoptosis accompanied by differentiation process was obviously inhibited and cell viability was largely improved.Conclusion: With N-cad-Fc culture method,cell death ratio could be abased without affecting differentiation process.This method might provide us a low death ratio and high efficiency way for inducing cell differentiation.