Polysaccharide of Pueraria induced injury on PC12 cells

Objective:To examine the effects of polysaccharide of Pueraria in pheochromocytoma-derived PC12 cells. Methods: cytotoxic effects was assessed by measuring cell viability using the 3-4,5-dimethylthiazol-2-yl-2,5 diphenyltetrazolium （MTT） assay. Superoxide dismutase （SOD） was measured by xanthine-hypoxanthinee oxidase chemiluminescence system. nitrite production was measured by a Griess assay. Results: 0.01-1.0mg/ml polysaccharide inhibited cellular viability and strengthened the injury induced by H₂O₂ dose-dependently on PC12; with the increase of the concentration of polysaccharide,extracellular SOD was reduced while nitrite production was increased. Conclusions: polysaccharide of Pueraria can resulted in PC12 cells damage.