Different interventions inhibit the effects of angiotensin II on the cardiomyocyte viability

Objective: We investigated the inhibitory effects of AngII receptor antagonists Valsartan, CGP42112A and MAPK blocker PD098059 on the AngII-induced cardiac myocyte viability in order to observe whether different interferences can effectively influence the cellular energy metabolism and provide the new experimental evidence for the application of those agents in pharmacological therapy of congestive heart failure. Methods: Based on that the mitochondria of viable cells can act on MTT to produce formazan of the purple, we investigate the effect of AngII on the cultured myocyte viability and the intervention of Valsartan, CGP42112A and PD098059 by means of colorimetric method. Results: Our results showed that AngII caused the gradual increase of formazan optical density（OD） within definite concentration and time,and then decreased, even lower than the normal after about 24 hours. CGP42112A had no distinct effect of AngII on the cardiac myocyte viability, but Valsartan and PD098059 had the inhibiting effect, respectively. Conclusion: AngII-induced myocyte viability is mainly mediated by AT 1-Rand ERK is concerned with cellular energy metabolism.That has important meanings for the application of different interferences among the cellular signal transduction in pharmacological therapy of congestive heart failure in the future.