Effect of two cryoprotectants on expression and viability of α-actinin in human skin

Objective To compare the effects of different cryoprotectants on expression of α-actinin in human skin.MethodsFresh skin tissues were divided into control group,trehalose group,and DMSO group.DMSO and propylene glycol used as cryoprotectants were stored in liquid nitrogen at-196℃ for 7 days and re-warmed after 14 days.The structure of skins in different groups was compared with immunohistochemical staining.Oxygen consumption of skin grafts was measured.ResultsTrehalose and DMSO could protect skin tissues.The expression level of α-actinin in cryopreserved skin tissues was similar to that in fresh skin tissues.ConclusionThe protective effect of trehalose in combination with DMSO on skin tissues is better than traditional drugs.