Effect of ATM gene silence on growth of HepG2 cells

Objective To explore the effect of ATM gene silence on growth of HepG2 cells. Methods HepG2 cells infected with lentiviral vector contain ATM gene. RT-PCR was used to identify ATM gene expression after RNA interference. Then,growth of HepG2 cells was detected by MTT way. Results The expression level of ATM gene was similar between the group not infected with virus and control group. The expression level of ATM gene was lower in L1 and L2 groups than in group not infected with virus（P＜0.05） as well as in L2 group than in L1 group（P＜0.05）. The efficiency of ATM gene silence was higher in L2 group than in control group,accounting for 84.48% and 15.52%,respectively. The results showed that no significant change occurred in growth of HepG2 cells infected or not infected with virus after ATM gene silence. Conclusion The RNAi expression vector of slow virus siRNA in the ATM gene can be constructed. Growth of HepG2 cells is not inhibited after ATM gene is silenced by RNAi.