Investigation on early and non-hurt diagnosing bladder cancer by expressing of hTERT mRNA of exfoliated urorhelial cells

Objective: To study the mRNA of human telomerase reverse transcriptase（hTERT） in exfoliated urinary cells of bladder cancer and to evaluate its clinical significance.Methods: We examined the expression of mRNA of hTERT in exfoliated urorhelial cells of bladder and control by RT-PCR.Meantime,the telomere reoeat amplification protocol（TRAP） was performed for indentify the result of mRNA of hTERT.Results: The positive rate of hTERT mRNA of bladder cancer group is 93%（26/28） and control group is 10%（2/20）.The result was consistent with the TRAP.Conclusion: The result suggest the expression of hTERT mRNA can be useful in screeing for bladder cancer and diagnosing bladder cancer recrudesce after operation.