Osteocalcin genotypes detected by polymerase chain reaction-restriction fragment length po- lymorphism

Objective: To establish a simple,accurate and practical method for Osteocalcin （OC） genotype investigation Methods: OC gene was amplified by polymerase chain reaction（PCR） PCR products were digested with HindⅢ and subjected to PAGE and stained by silver nitrate The patterns of restriction fragment length polymorphism （RFLP） of OC gene were distinguished Results: The frequencies of genotype hh,Hh,HH were 64%,32% and 4% respectively in 77 healthy individuals Conclusion: PCR RFLP method is considered a simple and practical technique for obtaining accurate results in this research This method is suitable for routine laboratories and epidemiology studies on OC genotype determination