Expression of Foxp3,Galectin-9 and PD-L1 in mouse DC2.4 cell line

Objective To observe the expression of CD80,CD86,Foxp3,Galectin-9 and PD-L1 in mouse dendritic cells（DC） and to study the immunological characteristic of mouse DC2.4 cell line. Methods Morphology of mouse DC2.4 cells was observed under an optical microscope after the cells were cultured with RPMI-1640 culture medium containing 10% fetal calf serum（FCS）.Expressions of Foxp3,Galectin-9 and PD-L1 in DC cells were detected by flow cytometry（FCM）. Results Optical microscopy revealed that the DC2.4 cells grew along the wall of DC and were dendritic,spindle,and irregular in shape.Flow cytometry showed that 91.04%±4.90% of CD80 and 98.54%±1.96% of CD86 were expressed in DC2.4 cells,respectively.Foxp3 was not or seldom expressed,while 14.33%±2.94% of Galectin-9 and 98.80%±0.63% of PD-L1 were expressed,respectively,in DC2.4 cells. Conclusion CD80,CD86 and PD-L1 are highly expressed,Galectin-9 is slowly expressed,and Foxp3 is not or seldom expressed in DC2.4 cell line.