Effect of anisodamine on cardiopulmonary resuscitation and myocardial ultrastructure of rats

Objective To observe the effects of anisodamine（Ani） on cardiopulmonary resuscitation and myocardial ultra-structure of rats with cardiac arrest. Methods A rat model of cardiac arrest was established by electric shock.Forty-five healthy rats were randomly divided into normal saline group（control group）,epinephrine group（Epi group）,and Epi plus Ani group（Ani group）,15 rats in each group.Four minutes after cardiac arrest,respirator-aided respiration and cardiac massage were started.After another 4 minutes,control group received normal saline,Epi group was injected with Epi（200μg·kg^-1）,and Ani group was given Epi（200μg·kg^-1） plus Ani（10μg·kg^-1） to observe their restoration of spontaneous circulation（ROSC）,success rate of resuscitation,and myocardium structure. Results The ROSC and success resuscitation rate was 95% and 80% respectively in Ani group,75% and 46.7% respectively in Epi group,and 60% and 26.7% respectively in control group.Light and electron microscopy showed that the damage of myocardium was milder in Ani group than in Epi and control groups. Conclusion Ani used in early resuscitation can ameliorate the side effect of Epi,improve the damage of myocardium,and increase the success rate of resuscitation.