The evaluation of ELISA and protein chip assays in the diagnosis of coronavirus-associated SARS

Objective:To evaluate the methods of different ELISA-assays and the protein chip assays for measurement of serum SARS-CoV antibody and to study the clinical significance of serum antibody against SARS-CoV.Methods:Convalescent sera from 135 clinically confirmed SARS patients，and 500 sera of healthy population have been measured by two ELISA methods and 48 cases have been analyzed by multiple antigens protein chip technique.Results:the repeatability of two ELISA methods were 6.06%、10.5%（coefficient of variability within run ） and 6.56%、11.3%（coefficient of variability between days） respectively，Correlation coefficient of two ELISA methods was 0.9456，The positive rates of specific IgG against SARS virus were 70.83%、70.83% and 72.9% respectively which were tested by 48 confirmed SARS cases.Conclusion:ELISA method and protein chip assay can be used to assist diagnosis of SARS patients.The sensitivity of protein chip assay is better than ELISA methods.