The study of the production of nitric oxide in β-Amyloid1-42 stimulated microglia in vitro

Objective: To explore the production of nitric oxide（NO） in β-Amyloid1-42 stimulated microglia in vitro.（Methods: We） cultured murine microglia BV-2 cells as the model of microglia in vitro.The production of nitric oxide（NO） and the activity of inducible nitric oxide synthase（iNOS） were studied in Aβ 1-42 stimulated BV-2 microglia.iNOS protein expression was examined by flow cytometry.Results: NO production was induced by Aβ1-42 in a dose and time dependent manner,Aβ1-42 can also increase iNOS activity and iNOS protein expression in a dose and time dependent manner.iNOS maybe the main source of NO production.Conclusions: On exposure to Aβ microglia can secrete NO,which may trigger pathways of neuronal degeneration in Alzheimer’s disease.amyloid beta-protein;microglia;nitric oxide