Effects of monoclonal antibody against endotoxin on TNF, IL-1 and IL-6 in the septic rats

The clinical manifestations of sepsis are the expressions of systemic inflammatory response mediated by endogenous mediators and cytokines secreated by the infected individual himself. Based on this understanding, various new approaches of anti inflammatory therapies for interrupting the inflammatory cascade have been investgigated. This study was designed to evaluate the effects of monoclonal antibody against endotoxin M2B on TNF, IL-1, and IL-6 in the septic rats. Sepsis were produced in rats by method of cecal ligation and perforation （CLP）. Treatment animnals received M2B 2mg/kg prior to CLP. Results showed that serum TNF levels reduced from the sepsis group of 39.93±3.84 to 27.94±6.89%, （lysis P ＜0.01） in the treatment group, IL-1 from 142.25±27.46 to 67.33±27.35 kU/L（P ＜0.01）; and IL-6 from 25.27±6.40 to 8.77±2.97 kU/L （P ＜0.05）. It is thus concluded that monclonal antibody M2B may be a useful agent in fighting sepsis if properly used in selected patients.