Abstract: Chronic liver disease (CLD) is a public health issue with high incidence, and it tends to impose an irreversible change on the structure and function of the liver. Its occurrence is accompanied by several kinds of metabolic abnormality, and glutamine metabolism plays an important role in the pathological progress of CLD. The regulation mechanism of signal pathways such as IL-6/STAT3/c-myc, PI3K/AKT/mTOR, ERK/NGFI-B/GLS1SIRT3/HIF-1α/GLS, Hedgehog-YAP and APC/C-Cdh1 for glutamine metabolism as well as the impact of glutamine metabolism on alcoholic liver disease, alcoholic fatty liver disease, fibrosis of liver, hepatoma and other chronic liver diseases are illustrated in this article, with the hope of providing a new vision to the fundamental research of CLD and to drug discovery.