Abstract: Surgical resection is the first choice for treatment of solid tumors, and accurate resection can effectively improve the prognosis of patients. Intraoperative fluorescence navigation technology is expected to guide accurate tumor resection intraoperatively. Indocyanine green (ICG) is the only near-infrared fluorescence agent approved for clinical use in China. Near-infrared-Ⅰ (NIR-Ⅰ) fluorescence navigation technique based on ICG has been used to guide the resection of various tumors, which is also proved to be of great value during the clinical application. Recently, it is found that ICG can be used for the near-infrared-Ⅱ (NIR-Ⅱ) fluorescence imaging, which further improves the quality of fluorescence imaging and the complete resection rate of the tumor. This paper summarizes and analyzes the clinical studies of fluorescence navigation technology based on ICG in guiding tumor resection intraoperatively, in order to provide suggestions for clinical treatment.