纳米石墨烯医用导电膏的制备及生物相容性评价

张艺耀; 赵永岐; 邹志康; 石进; 于东睿; 王雪峰; 陈大伟; 杨芬

doi:10.12435/j.issn.2095-5227.2023.072

纳米石墨烯医用导电膏的制备及生物相容性评价

Preparation and biocompatibility evaluation of nano graphene medical conductive paste

摘要

摘要:
背景医用导电膏作为连接介质，可以在皮肤和导联电极之间建立联系，以采集神经电信号、心电信号和肌电信号，临床上目前应用医用导电膏中存在一些缺陷，因此需要研发一种能够克服目前存在缺陷，且稳定性好、安全性高的医用导电膏。
目的探讨纳米石墨烯医用导电膏的制备方法并评价其生物相容性。
方法由纳米石墨烯、保湿剂、黏稠剂、表面活性剂、消毒组分、凝胶和磷酸盐缓冲液等原材料制备纳米石墨烯医用导电膏。测试其导电性、黏附性、阻抗性和信号稳定性。采用CCK-8实验评价其对表皮细胞增殖的影响和毒性，采用SD大鼠表皮评价其生物相容性。
结果纳米石墨烯医用导电膏的阻抗值、导电性、黏附性和信号稳定性等指标均优于传统医用导电膏技术指标(P＜0.05)。纳米石墨烯医用导电膏的生物相容性结果显示，其细胞增殖实验与空白对照组间差异无统计学意义(P＞0.05)。使用后大鼠表皮显示无皮肤发红、脱水现象发生，细胞和动物表皮组织的炎症因子指标显示与空白对照组间差异无统计学意义(P＞0.05)。
结论纳米石墨烯医用导电膏制备方法简单可行且具备良好生物相容性。

Abstract:
Background Medical conductive paste is used as a connecting medium to establish a connection between the skin and the lead electrode to collect nerve electrical signals, ECG signals and EMG signals. There are some defects in the current clinical application of medical conductive paste, so it is necessary to develop a medical conductive paste that can overcome the existing defects, with good stability and safety.
Objective To evaluate the preparation methods and biocompatibility of nano medical conductive paste.
Methods Nano graphene, humectant, viscous agent, surfactant, disinfection component, gel and phosphate buffer and other raw materials were prepared by homogeneous dispersion, grinding and stirring methods, and their conductivity, adhesion, signal stability and impedance were tested. CCK-8 experiment was used to evaluate the proliferation and toxicity of epidermal cells, and its biocompatibility was evaluated by SD rat epidermis.
Results The impedance value, conductivity, adhesion and signal stability of nano medical conductive paste were better than those of the traditional medical conductive paste. The cell proliferation test of nano medical conductive paste showed no statistical difference with the blank control group (P＞0.05), while the rat epidermal test showed no skin redness and dehydration, and the inflammatory factor index showed no significant difference with the control group (P＞0.05).
Conclusion The preparation method of nano medical conductive paste is feasible and the biocompatibility of nano medical conductive paste is good.

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