Abstract:
Background Smoke inhalation lung injury is common in burn patients. Inhaled toxic gases and particles are easy to cause acute lung injury (ALI), and there is no specific treatment at present.
Objective To investigate the effects of human amniotic mesenchymal stem cells (hAMSCs) on proliferation, apoptosis and inflammatory factors of alveolar type Ⅱ epithelial cells (AT-Ⅱ) in rats exposed to pine sawdust smoke solution.
Methods hAMSCs and AT-Ⅱ were isolated and cultured by enzyme digestion, and identified by flow cytometry and immunofluorescence, respectively; Cell damage induced by AT-Ⅱ replication smoke was exposed to pine sawdust smoke solution. The cells were divided into control group (serum-free DMEM/F12), injury group (exposure to 0.75‰ pine sawdust smoke solution) and treatment group (exposure to 0.75‰ pine sawdust smoke solution and hAMSCs co-cultured with AT-Ⅱ). CCK-8 assay was used to detect the proliferation of cells; Annexin V-FITC/PI staining was used to detect apoptosis; Elisa was used to analyze the expression of TNF-α, IL-6 and IL-10.
Results Compared with the control group, the activity of AT-Ⅱ in the injured group decreased (P<0.01), and after hAMSCs treatment, the activity of AT-Ⅱ cells increased compared with the injury group (P<0.01); The apoptosis rate in the injured group increased at 12 h and 24 h (P<0.01), while decreased after hAMSCs treatment (P<0.01); The contents of inflammatory factors TNF-α, IL-6 and IL-10 increased after cell injury (P<0.05), and the expression levels of pro-inflammatory factors TNF-α and IL-6 decreased (P<0.05) after 12 h and 24 h of co-culture with hAMSCs, and the content of IL-10 increased after co-cultured for 12 h and 24 h (P<0.05).
Conclusion hAMSCs can promote the proliferation and inhibit apoptosis of AT-Ⅱ induced by pine sawdust smoke solution, which may be related to regulating the expression of inflammatory factors, and provide theoretical reference for exploring the treatment of acute lung injury caused by smoke with hAMSCs.
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