Abstract:
Background Hepatocellular carcinoma, a common malignancy, involves complex signaling pathways critical for understanding its molecular mechanisms. Inhibiting these pathways can impede tumor growth and spread, suggesting new treatment approaches.
Objective To investigate the impact of circRNA-PTPRM on liver cancer cell proliferation, migration, and invasion.
Method The study involved detecting PTPRM and miR-139-5p levels in liver cancer cells using qRT-PCR. Bioinformatics and dual luciferase assays verified the interaction between PTPRM and miR-139-5p. Cell proliferation was assessed using MTT assay, while Transwell experiments evaluated cell migration and invasion. Western blotting analyzed protein expression related to cell proliferation and invasion.
Result PTPRM levels were higher and miR-139-5p levels lower in HepG2, Hep3B, and Huh-7 cell lines compared to normal liver HL-7702 cells (P<0.05). HepG2 was used for further experiments. Bioinformatics and experimental data confirmed PTPRM's regulatory effect on miR-139-5p expression. PTPRM upregulation negated miR-139-5p's suppressive effects on liver cancer cell proliferation, migration, and invasion. Conversely, silencing PTPRM increased miR-139-5p levels and reduced hepatocellular carcinoma cell proliferation, migration, invasion, and Cyclin D1 expression.
Conclusion Silencing PTPRM potentially elevates miR-139-5p expression, correlating with reduced proliferation, migration, and invasion of liver cancer cells.