Abstract: Abstract: Background There is a high incidence of hyperuricemia among residents in southern coastal areas under high temperature and high humidity environment. Objective To establish an animal model of hyperuricemia caused by high temperature and high humidity environment, and to explore the pathological changes in kidney, liver and ankle joints. Methods A total of 30 SPF-SD male rats were divided into following five groups: control group (CON, n=6), potassium oxonate group (PO, n=6), high purine diet group (HPD, n=6), high temperature-humidity group (HT, n=6), and compound factor group (CF, n=6). The ratsfromCON group were only given 0.5% sodium carboxymethyl cellulose solution by gavage. PO group was gavaged with 250mg/kg potassium oxonate suspension, while HPD group was gavaged with 250mg/kg potassium oxonate suspension and fed with 20% yeast feed. On the basis of the above intervention modeling methods for the PO group and the HPD group, HT group and CF group were treated in high temperature-humidity environment, that is, irradiated in a special constant temperature incubator with a temperature of 37±0.5℃ and a relative humidity of 80±1% for 1 h/d. The experiment lasted for 12 continuous weeks, atthetime of 0 week, 6th week and 12th week all rats were fasted for at least 12h before collecting blood samples so as to test their SUA levels. Three rats from each group were anesthetized to collect their renal, heart, liver and ankle tissues after modeling for 6 weeks and 12 weeks respectively. Results (1) the SUA levels of CF group, HT group, HPD group PO group are significantly higher than that of CON group (P＜0.05), and that of HT group was higher than PO group (P＜0.05), CF group was higher than HPD group (P＜0.05). (2) Inthisstudy, the renal tissue morphology of PO group and HPD group showed tubular dilatation, while there were a large number of epithelial cells of proximal convoluted tubules swelled in HT and CF groups, lead to the lumen gap narrowed, and some tubule spaces showed significant inflammatory infiltration. In addition, some renal tubular epithelial cells atrophy in CF group. (3) Under light microscope, the hepatic sinuses in PO group and HPD group were slightly dilated, while the liver cells in HT group and CF group were swollen and the hepatic sinuses were narrowed. In CF group, the arrangement of myocardial myofilaments was slightly disordered, and the structure of ankle cartilage layer was not smooth, but other tissue structures were not obviously abnormal.(4) At the 6th week of modeling, urate crystals were deposited in HPD group, HT group and CF group, among which CF group was the worst. And at the 12th week, the crystals in HPD group, HT group and CF group all decreased. Conclusions Hyperuricemia rat model prepared by 12-week high-temperature-humidity environment treatment presented extensive changes in the morphology of kidney, liver, heart and ankle joints.