Abstract: Objective: To understand the impact on the biology of K562 cell by the dominant expression of sphingosine kinase-1.Methods: K562 cells were separately infected with pLXSN and pLXSN-SphK recombinant retrovirus supernatant,and then selected for 1 000 ng/ml G418 resistance.The protein of well-growthing K562-pLXSN and K562-SphK cells were collected,and the intracellular SphK-1 activities were assayed.The proliferation status and the sensitivity to Gleevec of K562-SphK cells and K562-pLXSN cells were evaluated with MTT.Results: The activity of SphK-1 in K562-SphK cells was significantly higher than that in K562-pLXSN cells.There was no markedly difference in the proliferation status between K562-SphK cells and K562-pLXSN cells.K562-SphK cells were more sensitive to Gleevec than K562-pLXSN cells after Gleevec concentration at（0.5-10 μmol/L）（P＜0.05）.Conclusions: Dominant expression of SphK-1 in K562 cells didn’t affect cell proliferation,but increased the sensitivity to higher concentration of Gleevec.