不同浓度一氧化氮对5种细菌生长的影响

Effect of different concentration NO on five bacteria's growth in vitro

摘要: 目的: 探讨一氧化氮（NO）对细菌的体外抑菌作用。方法: 表皮葡萄球菌、大肠杆菌、金黄色葡萄球菌、绿脓杆菌、白色念珠菌5种病菌,在温度37℃,湿度45%~60%条件下,分别暴露于室内空气及10×10^-6g/L、25×10^-6g/L、40×10^-6g/L NO中24 h。结果: 10×10^-6g/L、25×10^-6g/L、40×10^-6g/L NO抑制白色念珠菌生长,对金黄色葡萄球菌无抑制;25×10^-6g/L、40×10^-6g/L NO对表皮葡萄球菌、大肠杆菌有抑菌作用;绿脓杆菌在40×10^-6g/L NO中被抑制。结论:①NO对细菌具有选择性抑制作用。②不同细菌对NO敏感性不同,NO对部分细菌抑菌作用呈浓度依赖性。

Abstract: Ojbective: To investigate the bacteriostatic effect of NO on bacteria's growth in vitro.Methods: Staphylococcus epidermidis,colibacillus,staphylococcus aureus,pseudomonas aeruginosa and Candida albicans were cultured in NO with air and 10×10^-6g/L、 25×10^-6g/L、 40×10^-6g/L NO,placed in cabinet with 37℃ and 45%～60% percent humidity for 24 hours.Results: 10×10^-6g/L、 25×10^-6g/L、 40×10^-6g/L NO had the effect of bacteriostasis on Candida albicans,but no effect on Staphylococcus aureus.25×10^-6g/L、 40×10^-6g/L NO had the effect on staphylococcus epidermidis and colibacillus.Pseudomonas aeruginosa was restrained by 40×10^-6g/L NO.Conclusions: ①NO has bacteriostasis effect on bacteria.②The bacteriostasis effect of NO is concentration dependent.