分子标志物在SGC-7901肿瘤干细胞相关亚群中的表达

李亚卓; 赵坡; 吕亚莉; 钟梅; 刘林

doi:10.3969/j.issn.1005-1139.2008.02.019

分子标志物在SGC-7901肿瘤干细胞相关亚群中的表达

Study on expression of molecular markers in cancer stem cell related subpopulation of cell line SGC-7901

摘要

摘要: 目的: 寻找胃癌SGC-7901细胞株中SP(side population)亚群细胞的特异性标志物。方法: 用Hoe-chest33342染色检测SGC-7901细胞株中SP细胞的比例;通过荧光免疫细胞化学染色比较DNA-PKcs、FHIT、Id2、NFκB/p65及p16在SP细胞与非SP细胞中表达的差异。结果: 胃癌SGC-7901细胞中SP细胞的比例为3.8%;FHIT、Id2及p16在SP细胞中的阳性表达率显著低于在非SP细胞中的阳性表达率(P<0.001),DNA-PKcs、NFκB/p65在SP细胞和非SP细胞中的阳性表达率无显著性差异。结论:FHIT、Id2及p16可能是胃癌SGC-7901癌干细胞的特异性分子标志物,而DNA-PKcs、NFκB/p65在鉴定胃癌干细胞时无特异性。

Abstract: Ojbective: To explore specific molecular markers in side population of gastric carcinoma cell line SGC-7901.Methods: The percentage of SP cells was detected in gastric carcinoma cell line SGC-7901 by using Hoechst33342 staining,and the expression of DNA-PKcs,FHIT,Id2,NFκB/p65 and p16 in SP cells were compared with those in non-SP cells through fluorescence immunocytochemical method.Results: The percentage of SP cells in gastric carcinoma cell line SGC-7901 was 3.8%.The positive expression rates of FHIT,Id2 and p16 in SP cells were significantly lower than those in non-SP cells（P＜0.001）.There was no markedly difference in the positive expression rates of DNA-PKcs and NFκB/p65 between SP cells and non-SP cells.Conclusions: FHIT,Id2 and p16 may be specific molecular markers to cancer stem cells in gastric carcinoma cell line SGC-7901,while DNA-PKcs and NFκB/p65 may not relate to cancer stem cell.

HTML全文

参考文献(0)

施引文献

资源附件(0)