Abstract: Objective To study the application of cortical electrode,image fusion and intra-operative MRI navigation in surgical resection of epileptic lesions in the functional area. Methods Application of cortical electrode,image fusion and intra-operative MRI navigation in surgical resection of epileptic lesions in 25 patients with refractory epilepsy was retrospectively analyzed.Intracranial cortical electrodes were implanted into epileptic lesions of the patients according to their VEEG during the first operation.Their cortical electrical activity was monitored,cerebral motor area was located by cortical electrical stimulation,a figure of correlation between epileptic lesion and functional area was plotted after operation,images of CT,MRI,MEG or PET were fused,and the epilepsy lesions were precisely removed under microscopic MRI navigation. Results Cortical electrical stimulation was successful in 25 epilepsy patients during the first operation and the cortical motor area around the epileptic lesion was accurately located.The images of MRI,MEG or PET for the patients were fused.Of the 25 patients,20 underwent total resection of epileptic lesion and 5 underwent partial resection of the lesion under microscopic MRI navigation due to the overlapped lesion and functional area.The residual cortex was eradicated by thermal burn.Transient opposite extremity dysfunction was observed in 1 patient after operation.No permanent neurological deficit occurred in all patients after operation. Conclusion Accurate location of intracranial cortical electrodes is of great importance for monitoring epileptic lesions.Fusion of MRI,MEG or PET images and microscopic MRI navigation during operation can provide technical support for the precise resection of epileptic lesions and protection of the functional area.