Abstract: Objective To study the nursing methods of patients with severe aplastic anemia（SAA） after transplantation of haploid hematopoietic stem cells（haplo-HSC）. Methods Toxic reaction to antithymocyte globulin（ATG） in SAA patients was observed,transfusion speed and time of bone marrow stem cells and umbilical cord-derived mesenchymal stem cells（UC-MSC） were strictly controlled,and measures were taken to prevent graft versus host disease（GVHD）. Results No toxic reaction to ATG occurred in the 5 SAA patients.Bone marrow stem cells and haplo-HSC were successfully transfused into the patients with no hemolytic and blood transfusion reaction.Heterogeneous hematopoiesis was reconstructed in all patients.Skin rejection occurred in 3 patients（2 of them were complicated by liver rejection） and intestinal rejection occurred in 1 patient.The patients survived disease-free at the end of follow-up except 1 patient who was discharged 78 days after transplantation and lost contact. Conclusion Effective nursing for patients with GVHD after transplantation is the key to the success of transplantation.