Abstract: Objective To provide the scientific evidence for the control of hospital infection with imipenem-resistant Acinetobacter baumannii and its epidemiological survey by investigating the prevalence of its OXA genotypes in our hospital. Methods The OXA genetypes of 68 imipenem-resistant Acinetobacter baumannii strains isolated in our hospital from April 2010 to December 2010 were analyzed by multiplex PCR. Results Of the 68 isolated imipenem-resistant Acinetobacter baumannii strains, 7 were OXA-51 positive gene with a detection rate of 10.29%, 60 were OXA-51 and OXA-23 positive gene with a detection rate of 88.24%, and 1 was OXA-51 and OXA-58 positive gene with a detection rate of 1.47%. No OXA-24 gene was detected. Conclusion OXA-51+OXA-23 genotype is the popular OXA genetype of imipenem-resistant Acinetobacter baumannii in our hospital, indicating that it is the most important mechanism underlying the resistance of imipenem-resistant Acinetobacter baumannii to carbapenemases in our hospital, and laboratory should strengthen its monitoring of enzyme-producing strains.