Abstract: Objective To investigate the distribution of aerobic and facultative anaerobe pathogens in patients with intra-abdominal infections in our hospital from January 2011 to May 2012 and their in vitro drug susceptibility. Methods Bacteria were identified using the VITEK COMPACT system. Their drug susceptibility was detected with microdilution method and analyzed with the WHONET5.5 software. Results Of the 111 isolated pathogens, 70.3%(78/111) were Gram-negative and 29.7%(33/111) were Grampositive. The top 7 pathogens were Escherichia coli (21.6%), Acinetobacter baumannii 18.0%), Enterococcus faecium (16.2%), Klebsiella pneumoniae (6.3%), Pseudomonas aeruginosa(6.3%), Enterococcus faecalis (6.3%), and Staphylococcus aureus(4.5%).The susceptibility of Enterobacteriaceae to imipenem, ertapenem, amikacin, piperacillin-tazobactam and cefotetan 100%, 100%, 97.7%, 90.7% and 90.7%, respectively. The drug resistance rate of Acinetobacter baumannii was the highest. The proportion of multidrug resistant (MDR) strains and extensive drug resistant (XDR) strains of Acinetobacter baumannii was 90% and 60%, respectively. The multidrug resistant rate of Pseudomonas aeruginosa was 14.3%. All Enterococcus faecium and Enterococcus faecalis were susceptible to vancomycin, linezolid and tigecycline. The drug resistant rate of Enterococcus faecium is higher than that of Enterococcus faecalis. No vancomycin resistant Staphylococcus aureu were found. Conclusion Intra-abdominal infection pathogens in our hospital are Gram-negative bacilli especially Enterobacteriaceae. Acinetobacter baumannii are the important hospital acquired pathogen with a high drug resistance rate. Enterococcus faecium are the most common Gram-positive pathogen of intra-abdominal infections. The drug resistance of bacteria is still severe, more effective measures should be taken to control the prevalence of drug resistant strains.