Abstract: Objective To provide the evidence for treatment of Staphylococcus aureus infection by investigating its drug resistance in 2003-2011. Methods Blood samples of Staphylococcus aureus were taken using the blood culture bottle provided by Beijing Botai Technique Development Center and cultured in BacT/ALERT 3D automated blood culture system. The isolated bacteria were identified with the VITEK system, their drug sensitivity was tested by BIOMIC and their drug resistance was analyzed by WHONET 5.4 software. Results Of the 155 Staphylococcus aureus strains isolated from January 2003 to December 2011, 110 were methicillinresistant S.aureus (MRSA), accounting for 70.9%. No resistance of Staphylococcus aureus to LNZ and VAN was found. The resistance of Staphylococcus aureus was below 30% to NIT and SXT, above 50% to TCY, LVX, CLX, CIP, CZO, AZM, ERY and PEN, 21.6% to 7 commonly used antibiotics and 34.6% to 8 commonly used antibiotics, respectively. Conclusion Staphylococcus aureus isolated from blood culture is highly resistant to drugs. The MRSA is resistant to multiple drugs and its drug resistance monitoring should be strengthened.