Abstract: Objective To investigate the distribution of the major hospital infection bacteria in our hospital from 2009 to 2011 and their drug resistance monitoring. Methods Bacteria in 15 868 samples from patients admitted to our hospital from January 2009 to December 2011 were cultured. The isolated bacteria were identified with Microscan-walkaway 96 and their drug sensitivity was analyzed. Results One hundred and forty-two kinds of bacteria including 1 783 strains were identified, of which 1 443 (80.93%)were Gram-negative strains and 340 (19.07%) were Gram-positive strains. The most common bacteria were Klebsiella pneumoniae(K. pneumoniae) followed by Escherichia coli (E. coli), Pseudomonas aeruginosa (P. aeruginosa), Acinetobacter baumannii (A.baumanni), Staphylococcus aureus (S. aureus), Staphylococcus epidermidis (S. epidermidis), Bacillus cloacae and Enterococcus faecalis (E. faecalis). The 1 783 strains were mainly isolated from sputum followed by from urine, blood, throat swab, cerebrospinal fluid and pus. The common bacteria had a low sensitivity to several antibiotics. Different Enterobacteriaceae were most sensitive to imipenem and Gram-positive strains were most sensitive to vancomycin. Conclusion Gram-negative bacteria are the most common hospital infection pathogens in the recent 3 years, which are mainly distributed in the lower respiratory tract with a high drug resistance.