Abstract: Objective To observe the clinical therapeutic effect of arthroscopic transfix system + interference screw + spiked ligament staple fixation with autologous hamstring tendon on anterior cruciate ligament (ACL) reconstruction. Methods Fifty-six patients with ACL fragmentation admitted to our hospital from June 2010 to April 2011 underwent transfix system fixation at the femoral side，interference screw and spiked ligament staple fixation at the tibial side for single bundle ACL reconstruction with autologous hamstring tendon. Their clinical therapeutic effects were observed by knee motion test, anterior drawer test (ADT), Lachman test, pivot shift test, one-leg hop test, Lysholm score, and International Knee Documentation Committee(IKDC) 2000 subjective knee score and knee motion KT1000 arthrometry after operation. Results The patients were followed up for 13-24 months (average 15.6 months). Their Lysholm score and IKDC score were significantly higher after operation than before operation (92.34±3.84 vs 57.71±7.45, 90.23±5.14 vs 69.86±8.95, P< 0.001), whereas their KT1000 arthrometry length was significantly shorter at the last follow-up than before operation (2.72±1.12 mm vs 6.38±2.31 mm, P< 0.001). Conclusion Arthroscopic transfix system fixation with autologous hamstring tendon is a simple procedure for ACL reconstruction with a satisfactory short-term clinical curative effect, a reliable fixation of implants, and a rapid recovery of the patients after operation.