Abstract: Objective To provide the evidence for the rational use of antibiotics by investigating the distribution pseudomonas aeruginosa in our hospital from 2006 to 2011 and its drug resistance. Methods The detection rate, distribution characteristics and drug sensitivity test findings of pseudomonas aeruginosa isolated from a variety of clinical specimens were retrospectively analyzed. Results The detection rate of pseudomonas aeruginosa in sputum samples was the highest followed by in urine specimens, with a detection rate of 81.5% and 8.3%, respectively. The pseudomonas aeruginosa was most sensitive and resistant to polymyxin B followed by cefoperazone and sulbactam. Conclusion Highly drug resistant pseudomonas aeruginosa can cause infection in many other sites, especially in the upper respiratory tract. Antibiotics should be rationally used for pseudomonas aeruginosa infection and drug resistance of pseudomonas aeruginosa should be closely monitored in order to effectively control its infection.