重症急性胰腺炎大鼠血清Chemerin水平变化的实验研究

辛宪磊; 冯健; 陈永亮; 刘志伟; 李崇辉; 李鸾

doi:10.3969/j.issn.2095-5227.2013.06.028

重症急性胰腺炎大鼠血清Chemerin水平变化的实验研究

Serum chemerin level in rats with severe acute pancreatitis

摘要

摘要: 目的探讨在重症急性胰腺炎(severe acute pancreatitis，SAP)大鼠血清中Chemerin、C反应蛋白(C-reative protein，CRP)、肿瘤坏死因子α(TNF-α)水平的变化。方法胆胰管内注射5%牛黄胆酸钠造模。将动物随机分为对照组、假手术组、重症急性胰腺炎组，各组于术后12 h时间点处死动物，处死前抽血测血清淀粉酶、Chemerin、TNF-α、CRP水平，同时取处死大鼠的胰腺组织，测定胰腺/体重比值，行HE染色，观察大鼠胰腺组织病理变化。结果 SAP组血清Chemerin、CRP、TNF-α水平较正常对照组、假手术组均明显升高(P<0.01)。血清Chemerin、CRP、TNF-α水平改变与胰腺组织病理评分呈正相关(r=0.802、0.785，0.769，P<0.01)。结论 Chemerin可能与重症急性胰腺炎的发生、发展有关，可作为早期预测急性胰腺炎严重程度的有效指标之一。

Abstract: Objective To study the serum levels of chemerin, CRP and TNF-αin rats with severe acute pancreatitis (SAP). Methods A SAP model was established by injecting 5% sodium taurocholate into the cholangiopancreatic duct.Thirty rats were randomly divided into control group, sham operation group) and SAP group (10 in each group).The rats were killed at 12 h after operation.Their serum levels of amylase, chemerin, CRP and TNF-αwere measured.The pancreas/BMI ratio was calculated.Lesions in pancreatic tissue of rats were observed with HE staining. Results The serum levels of chemerin, TNF-α, and CRP were signif icantly higher in SAP group than in control group and sham operation group (P< 0.01) and positively related with the pathological pancreatic tissue score(r=0.802, 0.785, 0.769, P< 0.01). Conclusion Chemerin may be related with the occurrence and progress of SAP and can be used as one of the important indications for predicting the severity of early SAP.

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