睾酮对体外培养小鼠卵巢颗粒细胞分泌抗苗勒管激素的影响
Effect of testosterone on secretion of anti-Mullerian hormone in mouse ovarian granular cells cultured in vitro
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摘要: 目的 探讨睾酮对体外培养的小鼠卵巢颗粒细胞抗苗勒管激素(anti-Mullerian hormone,AMH)分泌的影响。 方法 小鼠卵巢颗粒细胞原代培养,添加不同浓度睾酮,ELISA法检测颗粒细胞AMH的表达。 结果 ELISA法证实颗粒细胞中有AMH表达,不同浓度(10-8 mmol/L、10-7 mmol/L、10-6 mmol/L)睾酮作用24 h可促进颗粒细胞分泌AMH,且随睾酮浓度的增加、作用时间延长,AMH分泌明显增加(P<0.01)。 结论 睾酮促进小鼠卵巢颗粒细胞分泌AMH,高浓度比低浓度作用更强。Abstract: Objective To study the effect of testosterone on secretion of anti-Mullerian hormone(AMH) in mouse ovarian granular cells cultured in vitro. Methods Primary mouse ovarian granular cells were cultured in vitro and treated with testosterone at different concentrations. Expression of AMH in mouse ovarian granular cells was detected by ELISA. Results ELISA showed that the AMH was expressed in mouse ovarian granular cells. Twenty-four hours treatment of primary mouse ovarian granular cells with testosterone at the concentrations of 10-8 mmol/L, 10-7 mmol/L and 10-6 mmol/L up-regulated their secretion of AMH in a concentration-and time-dependent manner(P<0.01). Conclusion Testosterone up-regulates the secretion of AMH in mouse ovarian granular cells in a concentration-dependent manner.
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