Abstract: Objective To study the value of gated myocardial perfusion imaging (GMPI) in diagnosis and estimation of coronary atherosclerotic heart disease. Methods Sixty patients with suspected coronary heart disease (CHD) admitted to our department in 2012 underwent GMPI and coronary angiography (CAG) and other tests 1 week after GMPI to observe their left ventricular myocardial perfusion. Semi-quantitative score of GMPI was recorded by semi-quantitative analysis and its correlation with coronary Gensini score (GS) was analyzed. Results The sensitivity, specifcity and accuracy of GMPI in diagnosis of CHD were 93.33%, 80.00% and 90.00%, respectively. The sensitivity of GMPI in diagnosis of single lesion, double lesions and triple lesions was 75.00% (9/12), 82.35% (14/17) and 93.75% (15/16), respectively (χ²=21.794，P＜ 0.01). CAG showed 94 abnormal blood vessels in 45 patients. Of these 94 abnormal blood vessels, 34 showed mild stenosis, 38 showed moderate stenosis and 22 showed severe stenosis. The sensitivity of GMPI in diagnosis of the 3 kinds of stenosis was 76.47% (26/34), 86.84%(33/38), and 95.45% (21/22), respectively (χ²=98.55，P＜ 0.01). The semi-quantitative score of GMPI increased with the coronary GS. Pearson correlation analysis showed that the correlation coeffcient of mild stenosis, moderate stenosis, and severe stenosis was 0.751, 0.816, and 0.857, respectively (P＜ 0.01). Conclusion GMPI plays an important role in diagnosis of CHD. The severity of myocardial perfusion injury is positively related with the severity of CHD.