微量淋巴细胞毒技术与核酸扩增荧光技术检测HLA-B27的对比
Micro-lymphocytotoxicity test and PCR-dye assay in detection of HLA-B27:A comparative study
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摘要: 目的 通过比较核酸扩增荧光技术 (PCR-染料法) 和微量淋巴细胞毒技术在检测HLA-B27中的符合率, 分析两种方法的优势与不足, 探讨核酸扩增荧光技术 (PCR-染料法) 在HLA-B27检测中的临床应用价值。 方法 随机选取197例来我科就诊的疑似强直性脊柱炎 (ankylosing spondylitis, AS) 患者的样本, 分别使用微量淋巴细胞毒法和PCR-染料法检测。并对两种方法检测结果不符的样本进行测序复核。 结果 197例样本中, 微量淋巴细胞毒法检出HLA-B27阳性150例, 阴性47例;PCR-染料法检出阳性135例, 阴性62例;两种方法检测结果有统计学差异 (P＜0.01) 。15例差异样本, 两种方法与测序法相比较的符合率, 微量淋巴细胞毒法为13.3%, PCR-染料法为86.7%。 结论 核酸扩增荧光技术 (PCR-染料法) 和微量淋巴细胞毒法比较, 前者具有更高的临床准确性。在样本处理上PCR-染料法也更具优势, 操作简单方便, 有很强的临床应用价值。Abstract: Objective To study the application of PCR-dye assay and micro-lymphocytotoxicity test in detecting HLA-B27 by analyzing their advantages and disadvantages. Methods HLA-B27 was detected in 197 patients with ankylosing spongylitis（AS） by PCR-dye assay and micro-lymphocytotoxicity test,respectively.The inconsistent findings in PCR-dye assay and microlymphocytotoxicity test were tested by sequencing. Results Micro-lymphocytotoxicity test showed positive and negative HLAB27 in 150 and 47 out of the 197 patients.PCR-dye assay displayed positive and negative HLA-B27 in 135 and 62 out of the 197 patients（P＜0.01）.The HLA-B27 detection rate was significantly higher by micro-lymphocytotoxicity test than by PCR-dye assay in 15 patients with different findings（86.7% vs 13.3%,P＜0.05）. Conclusion The accuracy of PCR-dye assay is better than that of micro-lymphocytotoxicity test in detecting HLA-B27 and is advantageous over micro-lymphocytotoxicity test in sample processing and easier to perform,thus having a bright future in clinical application.
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