Abstract: Objective To detect the methylation of PCDH10 gene in prostate cancer (PCa) tissue and analyze its clinical significance. Methods Methylation of PCDH10 gene was detected in 3 PCa cell lines, 1 prostate epithelial cell line, 13 hyperplasic prostatic tissue samples and 40 PCa tissue samples by methylation-specific PCR. Clinical data about the 40 PCa patients were analyzed. Results Methylation of the PCDH10 gene was detected in 2 out of the 3 PCa cell lines and in 24 out of the 40 PCa tissue samples with a detection rate of 67.7% and 60.0%, respectively. However, no methylation of the PCDH10 gene was detected in the 13 hyperplasic prostatic tissue samples. No significant difference was found in the age, prostate specific antigen (PSA), and clinical stage between those with or without methylation of the PCDH10 gene (P< 0.05). The Gleason score was significantly higher in patients with methylation of the PCDH10 gene than in those without methylation of the PCDH10 gene (P< 0.05). Conclusion The incidence of PCDH10 gene methylation is rather high in PCa patients. The methylation rate of PCDH10 gene is related with the higher Gleason score, suggesting that PCDH10 gene methylation is related with the pathogenesis and progression of PCa.